ABflo® 488 Rabbit anti-Human Cytokeratin 8 mAb (100 T)

Brand: Abclonal
Cat. #: A23018
Availability: In Stock

$140.00

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Description

Reactivity:	Human
Applications:	FC (intra)
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Monoclonal Antibody
Conjugation:	ABflo® 488. Ex:491nm. Em:516nm.
Gene Name:	keratin 8
Gene Symbol:	KRT8
Synonyms:	K8; KO; CK8; CK-8; CYK8; K2C8; CARD2
Gene ID:	3856
UniProt ID:	P05787
Clone ID:	3E8W8
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-483 of human Cytokeratin 8 (NP_002264.1).
Dilution:	FC (intra),5 μl per 10^6 cells in 100 μl volume
Purification Method:	Affinity purification
Concentration:	0.04 mg/mL
Buffer:	PBS with 0.03% proclin300,0.2% BSA, pH7.3.
Storage:	Store at -20°C. Avoid freeze / thaw cycles.
Documents:	Manual-KRT8 Antibody

Background

This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene.

Images

	Flow cytometry:1×10^6 Jurkat cells (negative control,Left) and HeLa cells (Right) were intracellularly-stained with ABflo® 488 Rabbit anti-Human Cytokeratin 8 mAb(A23018,5 μl/Test,orange line) or ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,blue line). Non-fluorescently stained cells was used as blank control (red line).
	Flow cytometry:1×10^6 HeLa cells were intracellularly-stained with ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,left) or ABflo® 488 Rabbit anti-Human Cytokeratin 8 mAb(A23018,5 μl/Test,right).