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ABflo® 488 Rabbit anti-Human DNAJB1/HSP40 mAb (100 T)
| Reactivity: | Human |
| Applications: | FC (intra) |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal Antibody |
| Conjugation: | ABflo® 488. Ex:491nm. Em:516nm. |
| Gene Name: | DnaJ heat shock protein family (Hsp40) member B1 |
| Gene Symbol: | DNAJB1 |
| Synonyms: | Hdj1; Sis1; HSPF1; Hsp40; RSPH16B |
| Gene ID: | 3337 |
| UniProt ID: | P25685 |
| Clone ID: | 7T4E4 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 241-340 of human DNAJB1/HSP40 (NP_006136.1). |
| Dilution: | FC (intra),5 μl per 10^6 cells in 100 μl volume |
| Purification Method: | Affinity purification |
| Concentration: | 0.04 mg/mL |
| Buffer: | PBS with 0.03% proclin300,0.2% BSA, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-DNAJB1 Antibody |
Background
This gene encodes a member of the DnaJ or Hsp40 (heat shock protein 40 kD) family of proteins. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. The encoded protein is a molecular chaperone that stimulates the ATPase activity of Hsp70 heat-shock proteins in order to promote protein folding and prevent misfolded protein aggregation. Alternative splicing results in multiple transcript variants.
Images
 | Flow cytometry: 1×10^6 293T-DNAJB1/HSP40-shRNA-1 cells (pink line)、293T-DNAJB1/HSP40-shRNA-2 cells (green line) and 293T-scramble cells (orange line) were intracellularly-stained with ABflo® 488 Rabbit anti-Human DNAJB1/HSP40 mAb (A25527,5 μl/Test) or ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line). |
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