ABflo® 488 Rabbit anti-Human Glypican 3 (GPC3) mAb (100 T)

Brand: Abclonal
Cat. #: A22631
Availability: In Stock

$290.00

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Description

Reactivity:	Human
Applications:	FC
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Monoclonal Antibody
Conjugation:	ABflo® 488. Ex:491nm. Em:516nm.
Gene Name:	glypican 3
Gene Symbol:	GPC3
Synonyms:	SGB; DGSX; MXR7; SDYS; SGBS; OCI-5; SGBS1; GTR2-2
Gene ID:	2719
UniProt ID:	P51654
Clone ID:	1H6V5
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 481-580 of human Glypican 3 (GPC3) (NP_004475.1).
Dilution:	FC,5 μl per 10^6 cells in 100 μl volume
Purification Method:	Affinity purification
Concentration:	0.04 mg/mL
Buffer:	PBS with 0.03% proclin300,0.2% BSA, pH7.3.
Storage:	Store at -20°C. Avoid freeze / thaw cycles.
Documents:	Manual-GPC3 Antibody

Background

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The protein encoded by this gene can bind to and inhibit the dipeptidyl peptidase activity of CD26, and it can induce apoptosis in certain cell types. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome, also known as Simpson dysmorphia syndrome. Alternative splicing results in multiple transcript variants.

Images

	Flow cytometry:1×10^6 K-562 cells (negative control,Left) and HepG2 cells (Right) were surface-stained with ABflo® 488 Rabbit anti-Human Glypican 3 (GPC3) mAb(A22631,5 μl/Test,orange line) or ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line).
	Flow cytometry:1×10^6 HepG2 cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,left) or ABflo® 488 Rabbit anti-Human Glypican 3 (GPC3) mAb(A22631,5 μl/Test,right).