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ABflo® 488 Rabbit anti-Human IL9 mAb (100 T)
| Reactivity: | Human |
| Applications: | IF/ICC,FC (intra) |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal Antibody |
| Conjugation: | ABflo® 488. Ex:491nm. Em:516nm. |
| Gene Name: | interleukin 9 |
| Gene Symbol: | IL-9 |
| Synonyms: | P40; HP40; IL-9 |
| Gene ID: | 3578 |
| UniProt ID: | P15248 |
| Clone ID: | 4T5H8 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 19-144 of human IL9(NP_000581.1). |
| Dilution: | IF/ICC,1:50 - 1:200|FC (intra),5 μl per 10^6 cells in 100 μl volume |
| Purification Method: | Affinity purification |
| Concentration: | 0.04 mg/mL |
| Buffer: | PBS with 0.03% proclin300,0.2% BSA, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-IL-9 Antibody |
Background
The protein encoded by this gene is a cytokine that acts as a regulator of a variety of hematopoietic cells. This cytokine stimulates cell proliferation and prevents apoptosis. It functions through the interleukin 9 receptor (IL9R), which activates different signal transducer and activator (STAT) proteins and thus connects this cytokine to various biological processes. The gene encoding this cytokine has been identified as a candidate gene for asthma. Genetic studies on a mouse model of asthma demonstrated that this cytokine is a determining factor in the pathogenesis of bronchial hyperresponsiveness.
Images
 | Confocal imaging of 293T cells transfected with IL9 using ABflo® 488 Rabbit anti-Human IL9 mAb (A24306, dilution 1:50). DAPI was used for nuclear staining (Blue). Objective: 100x. |
 | Flow cytometry:1×10^6 293T cells (negative control,left) and 293T(Transfection,right) cells were intracellularly-stained with ABflo® 488 Rabbit anti-Human IL9 mAb(A24306,5 μl/Test,orange line) or ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line). |
 | Flow cytometry:1×10^6 293T(Transfection) cells were intracellularly-stained with ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,left) or ABflo® 488 Rabbit anti-Human IL9 mAb(A24306,5 μl/Test,right). |
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