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ABflo® 488 Rabbit anti-Human/Monkey Ki67 mAb (100 T)
| Reactivity: | Human |
| Applications: | FC (intra) |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal Antibody |
| Conjugation: | ABflo® 488. Ex:491nm. Em:516nm. |
| Gene Name: | marker of proliferation Ki-67 |
| Gene Symbol: | MKI67 |
| Synonyms: | KIA; MIB-; MIB-1; PPP1R105 |
| Gene ID: | 4288 |
| UniProt ID: | P46013 |
| Clone ID: | 10C3V7 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 2271-2370 of human Ki67 (NP_002408.3). |
| Dilution: | FC (intra),5 μl per 10^6 cells in 100 μl volume |
| Purification Method: | Affinity purification |
| Concentration: | 0.02 mg/mL |
| Buffer: | PBS with 0.03% proclin300,0.2% BSA, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-MKI67 Antibody |
Background
Enables protein C-terminus binding activity. Involved in regulation of chromosome segregation and regulation of mitotic nuclear division. Located in chromosome; nuclear body; and nucleolus. Colocalizes with condensed chromosome. Implicated in Crohn's disease; breast cancer; human immunodeficiency virus infectious disease; and pancreatic cancer. Biomarker of several diseases, including Barrett's esophagus; autoimmune disease of musculoskeletal system (multiple); endocrine gland cancer (multiple); gastrointestinal system cancer (multiple); and interstitial cystitis.
Images
 | Flow cytometry:1×10^6 knockout (KO) HeLa cells(negative control,left) and HeLa cells (right) were intracellularly-stained with ABflo® 488 Rabbit anti-Human/Monkey Ki67 mAb(A23407,5 μl/Test,orange line) or ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,blue line).Non-fluorescently stained cells were used as blank control (red line). |
 | Flow cytometry:1×10^6 HeLa cells were intracellularly-stained with ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,left) or ABflo® 488 Rabbit anti-Human/Monkey Ki67 mAb(A23407,5 μl/Test,right). |
 | Flow cytometry: 1×10^6 Human PBMC were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,left) or ABflo® 488 Rabbit anti-Human/Monkey Ki67 mAb (A23407,5 μl/Test,right). Cells in the lymphocyte gate were used for analysis. |
 | Flow cytometry: 1×10^6 Monkey PBMC were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,left) or ABflo® 488 Rabbit anti-Human/Monkey Ki67 mAb (A23407,5 μl/Test,right). Cells in the lymphocyte gate were used for analysis. |
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