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ABflo® 488 Rabbit anti-Human NG2 mAb (100 T)
| Reactivity: | Human |
| Applications: | FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal Antibody |
| Conjugation: | ABflo® 488. Ex:491nm. Em:516nm. |
| Gene Name: | chondroitin sulfate proteoglycan 4 |
| Gene Symbol: | CSPG4 |
| Synonyms: | NG2; MCSP; MCSPG; MSK16; CSPG4A; HMW-MAA; MEL-CSPG |
| Gene ID: | 1464 |
| UniProt ID: | Q6UVK1 |
| Clone ID: | 9Q1C9 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1583-2224 of human NG2 (NP_001888.2). |
| Dilution: | FC,5 μl per 10^6 cells in 100 μl volume |
| Purification Method: | Affinity purification |
| Concentration: | 0.02 mg/mL |
| Buffer: | PBS with 0.03% proclin300,0.2% BSA, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-CSPG4 Antibody |
Background
A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells.
Images
 | Flow cytometry: 1×10^6 Jurkat cells (negative control,left) and SK-MEL-28 (right) cells were surface-stained with ABflo® 488 Rabbit anti-Human NG2 mAb (A24978,5 μl/Test,orange line) or ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line). |
 | Flow cytometry: 1×10^6 SK-MEL-28 cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,left) or ABflo® 488 Rabbit anti-Human NG2 mAb (A24978,5 μl/Test,right). |
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