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ABflo® 488 Rabbit anti-Mouse CD11a mAb (100 T)
| Reactivity: | Mouse |
| Applications: | FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal Antibody |
| Conjugation: | ABflo® 488. Ex:491nm. Em:516nm. |
| Gene Name: | integrin alpha L |
| Gene Symbol: | Itgal |
| Synonyms: | Cd11a; LFA-1; Ly-15; Ly-21; (p180); LFA-1A |
| Gene ID: | 16408 |
| UniProt ID: | P24063 |
| Clone ID: | 2M8S10 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 149-340 of mouse CD11a (NP_001240801.1). |
| Dilution: | FC,5 μl per 10^6 cells in 100 μl volume |
| Purification Method: | Affinity purification |
| Concentration: | 0.02 mg/mL |
| Buffer: | PBS with 0.03% proclin300,0.2% BSA, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-Itgal antibody |
Background
Predicted to enable ICAM-3 receptor activity and cell adhesion molecule binding activity. Involved in cell-cell adhesion. Acts upstream of or within several processes, including activated T cell proliferation; positive regulation of T cell proliferation; and positive regulation of calcium-mediated signaling. Located in cell-cell junction; external side of plasma membrane; and immunological synapse. Is expressed in heart; hemolymphoid system; and vault of skull. Orthologous to human ITGAL (integrin subunit alpha L).
Images
 | Flow cytometry: 1×10^6 NIH/3T3 cells (Low Expression,left) and C57BL/6 mouse splenocytes (right) were surface-stained with ABflo® 488 Rabbit anti-Mouse CD11a mAb (A25827,5 μl/Test,orange line) or ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line). |
 | Flow cytometry: 1×10^6 C57BL/6 mouse splenocytes were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,left) or ABflo® 488 Rabbit anti-Mouse CD11a mAb (A25827,5 μl/Test,right). |
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