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ABflo® 488 Rabbit anti-Mouse CD14 mAb (100 T)
| Reactivity: | Mouse |
| Applications: | FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal Antibody |
| Conjugation: | ABflo® 488. Ex:491nm. Em:516nm. |
| Gene Name: | CD14 antigen |
| Gene Symbol: | Cd14 |
| Synonyms: | N/A |
| Gene ID: | 12475 |
| UniProt ID: | P10810 |
| Clone ID: | 8C9M3 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 18-345 of mouse CD14 (NP_033971.1). |
| Dilution: | FC,5 μl per 10^6 cells in 100 μl volume |
| Purification Method: | Affinity purification |
| Concentration: | 0.02 mg/mL |
| Buffer: | PBS with 0.03% proclin300,0.2% BSA, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-Cd14 Antibody |
Background
This gene encodes a protein that plays an important role in the innate immune response and is expressed in monocyte/macrophage cells. This gene product acts as a co-receptor that binds several microbial and fungal molecules, including lipopolysaccharides (LPS). This proteins LPS-binding activity is enhanced by the LPS binding protein (LBP) to allow binding to the TLR4-MD-2 co-receptor complex. The product of this gene is found in two forms, either as a soluble protein or attached to the cell surface by a glycosylphosphatidylinositol anchor.
Images
 | Flow cytometry: 1×10^6 Neuro-2a cells (negative control,left) and RAW 264.7 cells (right) were surface-stained with ABflo® 488 Rabbit anti-Mouse CD14 mAb (A25190,5 μl/Test,orange line) or ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line). |
 | Flow cytometry: 1×10^6 RAW 264.7 cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,left) or ABflo® 488 Rabbit anti-Mouse CD14 mAb (A25190,5 μl/Test,right). |
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