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ABflo® 488 Rabbit anti-Mouse S100A9 mAb (100 T)
| Reactivity: | Mouse |
| Applications: | FC (intra) |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal Antibody |
| Conjugation: | ABflo® 488. Ex:491nm. Em:516nm. |
| Gene Name: | S100 calcium binding protein A9 (calgranulin B) |
| Gene Symbol: | S100a9 |
| Synonyms: | p14; Cagb; GAGB; L1Ag; BEE22; MRP14; 60B8Ag |
| Gene ID: | 20202 |
| UniProt ID: | P31725 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-113 of mouse S100a9 (NP_033140.1). |
| Dilution: | FC (intra),5 μl per 10^6 cells in 100 μl volume |
| Purification Method: | Affinity purification |
| Concentration: | 0.02 mg/ml |
| Buffer: | PBS with 0.09% Sodium azide,0.2% BSA, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-S100a9 antibody |
Background
Predicted to enable calcium ion binding activity and calcium-dependent protein binding activity. Involved in peptidyl-cysteine S-nitrosylation; positive regulation of blood coagulation; and regulation of translation. Acts upstream of or within several processes, including actin cytoskeleton reorganization; astrocyte development; and regulation of integrin biosynthetic process. Predicted to be located in cell junction; cytosol; and nucleoplasm. Predicted to be active in cytoplasm; extracellular space; and nucleus. Is expressed in several structures, including jaw; liver; otic capsule; skeleton; and spleen red pulp. Human ortholog(s) of this gene implicated in myocarditis. Orthologous to human S100A9 (S100 calcium binding protein A9).
Images
 | Flow cytometry: 1×10^6 RAW 264.7 cells (negative control,left) and C57BL/6 mouse splenocytes (right) were intracellularly-stained with ABflo® 488 Rabbit anti-Mouse S100A9 mAb (A26915,5 μl/Test,orange line) or ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line). |
 | Flow cytometry:1×10^6 C57BL/6 mouse splenocytes were intracellularly-stained with ABflo® 450 Rabbit anti-Mouse CD11b mAb (A26229,5 μl/Test) and ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,left) or ABflo® 488 Rabbit anti-Mouse S100A9 mAb (A26915,5 μl/Test,right). Cells in the lymphocyte gate were used for analysis. |
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