ABflo® 594 Rabbit anti-FITC/5-FAM/6-FAM mAb (100 T)

Background

FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins.

Images

	Flow cytometry:1×10^6 HT-29 cells (negative control,Left) and Raji cells (Right) were surface-stained with Mouse anti-Human CD86 mAb(5 μl/Test,orange line) or ABflo® 594 Mouse IgG1 isotype control (5 μl/Test,blue line),then stained with ABflo® 594 Rabbit anti-FITC/5-FAM/6-FAM mAb(A24355,5 μl/Test）,Non-fluorescently stained cells were used as blank control (red line).
	Flow cytometry:1×10^6 Raji cells were surface-stained with ABflo® 594 Mouse IgG1 isotype control (5 μl/Test,left) or Mouse anti-Human CD86 mAb(5 μl/Test,right)，then stained with ABflo®594 Rabbit anti-FITC/5-FAM/6-FAM mAb(A24355,5 μl/Test).

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