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ABflo® 594 Rabbit anti-Human CD235a/Glycophorin A mAb (100 T)
| Reactivity: | Human |
| Applications: | FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal Antibody |
| Conjugation: | ABflo® 594. Ex:588nm. Em:604nm. |
| Gene Name: | glycophorin A (MNS blood group) |
| Gene Symbol: | GYPA |
| Synonyms: | MN; GPA; MNS; GPSAT; PAS-2; CD235a; GPErik; HGpMiV; HGpMiXI; HGpSta(C) |
| Gene ID: | 2993 |
| UniProt ID: | P02724 |
| Clone ID: | 5O2A5 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 20-91 of human CD235a/Glycophorin A (NP_002090.4). |
| Dilution: | FC,5 μl per 10^6 cells in 100 μl volume |
| Purification Method: | Affinity purification |
| Concentration: | 0.04 mg/mL |
| Buffer: | PBS with 0.03% proclin300,0.2% BSA, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-GYPA Antibody |
Background
Glycophorins A (GYPA) and B (GYPB) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups. In addition to the M or N and S or s antigens that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include all the variants of the Miltenberger complex and several isoforms of Sta, as well as Dantu, Sat, He, Mg, and deletion variants Ena, S-s-U- and Mk. Most of the variants are the result of gene recombinations between GYPA and GYPB.
Images
 | Flow cytometry: 1×10^6 THP-1 cells (negative control,left) and HEL cells (right) were surface-stained with ABflo® 594 Rabbit anti-Human CD235a/Glycophorin A mAb (A25111,5 μl/Test,orange line) or ABflo® 594 Rabbit IgG isotype control (A23821,5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line). |
 | Flow cytometry: 1×10^6 HEL cells were surface-stained with ABflo® 594 Rabbit IgG isotype control (A23821,5 μl/Test,left) or ABflo® 594 Rabbit anti-Human CD235a/Glycophorin A mAb (A25111,5 μl/Test,right). |
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