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ABflo® 594 Rabbit anti-Mouse CD25 mAb (100 T)
| Reactivity: | Mouse |
| Applications: | FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal Antibody |
| Conjugation: | ABflo® 594. Ex:588nm. Em:604nm. |
| Gene Name: | interleukin 2 receptor, alpha chain |
| Gene Symbol: | Il2ra |
| Synonyms: | CD25; Il2r; Ly-43 |
| Gene ID: | 16184 |
| UniProt ID: | P01590 |
| Clone ID: | 2C8F10 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 22-236 of mouse CD25 (NP_032393.3). |
| Dilution: | FC,5 μl per 10^6 cells in 100 μl volume |
| Purification Method: | Affinity purification |
| Concentration: | 0.04 mg/mL |
| Buffer: | PBS with 0.03% proclin300,0.2% BSA, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-Il2ra antibody |
Background
Enables interleukin-2 binding activity and interleukin-2 receptor activity. Acts upstream of or within several processes, including Notch signaling pathway; activation-induced cell death of T cells; and regulation of T cell proliferation. Located in external side of plasma membrane. Is expressed in bone marrow. Used to study Sjogren's syndrome and inflammatory bowel disease. Human ortholog(s) of this gene implicated in immunodeficiency 41; lymphopenia; multiple sclerosis; type 1 diabetes mellitus; and type 1 diabetes mellitus 10. Orthologous to human IL2RA (interleukin 2 receptor subunit alpha).
Images
 | Flow cytometry: 1×10^6 C2C12 cells(negative control,left) and CTLL-2 cells (right) were surface-stained with ABflo® 594 Rabbit anti-Mouse CD25 mAb(A23809,5 μl/Test,orange line) or ABflo® 594 Rabbit IgG isotype control (5 μl/Test,blue line).Non-fluorescently stained cells were used as blank control (red line). |
 | Flow cytometry:1×10^6 CTLL-2 cells were surface-stained with ABflo® 594 Rabbit IgG isotype control (5 μl/Test,left) or ABflo® 594 Rabbit anti-Mouse CD25 mAb(A23809,5 μl/Test,right). |
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