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ABflo® 610 Rabbit anti-Human CD94 mAb (100 T)
| Reactivity: | Human |
| Applications: | FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal Antibody |
| Conjugation: | ABflo® 610. Ex:421nm. Em:612nm. |
| Gene Name: | killer cell lectin like receptor D1 |
| Gene Symbol: | KLRD1 |
| Synonyms: | CD94 |
| Gene ID: | 3824 |
| UniProt ID: | Q13241 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 32-179 of human CD94. (NP_002253.2). |
| Dilution: | FC, 5 μl per 10^6 cells in 100 μl volume |
| Purification Method: | Affinity purification |
| Concentration: | 0.02 mg/ml |
| Buffer: | PBS with 0.09% Sodium azide,0.2% BSA, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-KLRD1 Antibody |
Background
Natural killer (NK) cells are a distinct lineage of lymphocytes that mediate cytotoxic activity and secrete cytokines upon immune stimulation. Several genes of the C-type lectin superfamily, including members of the NKG2 family, are expressed by NK cells and may be involved in the regulation of NK cell function. KLRD1 (CD94) is an antigen preferentially expressed on NK cells and is classified as a type II membrane protein because it has an external C terminus. Several transcript variants encoding different isoforms have been found for this gene.
Images
 | Flow cytometry: 1×10^6 HAP1 cells (negative control,left) and NK-92MI cells (right) were surface-stained with ABflo® 610 Rabbit anti-Human CD94 mAb (A26683,5 μl/Test,orange line) or ABflo® 610 Rabbit IgG isotype control (A25826,5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line). |
 | Flow cytometry: 1×10^6 NK-92MI cells were surface-stained with ABflo® 610 Rabbit IgG isotype control (A25826,5 μl/Test,left) or ABflo® 610 Rabbit anti-Human CD94 mAb (A26683,5 μl/Test,right). |
 | Flow cytometry: 1×10^6 Human PBMC were surface-stained with ABflo® 610 Rabbit anti-Human CD94 mAb (A26683,5 μl/Test,orange line) or ABflo® 610 Rabbit IgG isotype control (A25826,5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line).Cells in the Lymphocytes gate were used for analysis. |
 | Flow cytometry: 1×10^6 Human PBMC were surface-stained with ABflo® 610 Rabbit IgG isotype control (A25826,5 μl/Test,left) or ABflo® 610 Rabbit anti-Human CD94 mAb (A26683,5 μl/Test,right).Cells in the Lymphocytes gate were used for analysis. |
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