| Reactivity: | Human |
| Applications: | IF/ICC, FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal Antibody |
| Conjugation: | ABflo® 647. Ex:648nm. Em:664nm. |
| Gene Name: | CD24 molecule |
| Gene Symbol: | CD24 |
| Synonyms: | CD24A |
| Gene ID: | 100133941 |
| UniProt ID: | P25063 |
| Clone ID: | 6Q1G9 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-80 of human CD24 (NP_037362.1). |
| Dilution: | IF/ICC,1:50 - 1:200|FC,5 μl per 10^6 cells in 100 μl volume |
| Purification Method: | Affinity purification |
| Concentration: | 0.02 mg/mL |
| Buffer: | PBS with 0.03% proclin300,0.2% BSA, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-CD24 antibody |
Background
This gene encodes a sialoglycoprotein that is expressed on mature granulocytes and B cells and modulates growth and differentiation signals to these cells. The precursor protein is cleaved to a short 32 amino acid mature peptide which is anchored via a glycosyl phosphatidylinositol (GPI) link to the cell surface. This gene was missing from previous genome assemblies, but is properly located on chromosome 6. Non-transcribed pseudogenes have been designated on chromosomes 1, 15, 20, and Y. Alternative splicing results in multiple transcript variants.
Images
![]() | Confocal imaging of MCF7 cells using ABflo® 647 Rabbit anti-Human CD24 mAb (A25381, dilution 1:100). DAPI was used for nuclear staining (Blue). Objective: 100x. |
![]() | Flow cytometry: 1×10^6 293F cells (Low Expression,left) and MCF7 cells (right) were surface-stained with ABflo® 647 Rabbit anti-Human CD24 mAb (A25381,5 μl/Test,orange line) or ABflo® 647 Rabbit IgG isotype control (A22070,5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line). |
![]() | Flow cytometry: 1×10^6 MCF7 cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070,5 μl/Test,left) or ABflo® 647 Rabbit anti-Human CD24 mAb (A25381,5 μl/Test,right). |
![]() | Flow cytometry: 1×10^6 Human peripheral blood granulocytes were surface-stained with ABflo® 647 Rabbit anti-Human CD24 mAb (A25381,5 μl/Test,orange line) or ABflo® 647 Rabbit IgG isotype control (A22070,5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line). |
![]() | Flow cytometry: 1×10^6 Human peripheral blood granulocytes were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070,5 μl/Test,left) or ABflo® 647 Rabbit anti-Human CD24 mAb (A25380,5 μl/Test,right). |
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