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ABflo® 647 Rabbit anti-Human CD99 mAb (100 T)
| Reactivity: | Human |
| Applications: | IF/ICC, FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal Antibody |
| Conjugation: | ABflo® 647. Ex:648nm. Em:664nm. |
| Gene Name: | CD99 molecule (Xg blood group) |
| Gene Symbol: | CD99 |
| Synonyms: | MIC2; HBA71; MIC2X; MIC2Y; MSK5X |
| Gene ID: | 4267 |
| UniProt ID: | P14209 |
| Clone ID: | 4Z5F6 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 24-123 of human CD99 (NP_002405.1). |
| Dilution: | IF/ICC,1:50 - 1:200|FC,5 μl per 10^6 cells in 100 μl volume |
| Purification Method: | Affinity purification |
| Concentration: | 0.04 mg/mL |
| Buffer: | PBS with 0.03% proclin300,0.2% BSA, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-CD99 Antibody |
Background
The protein encoded by this gene is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. There is a related pseudogene located immediately adjacent to this locus.
Images
 | Confocal imaging of Hep G2 cells using ABflo® 647 Rabbit anti-Human CD99 mAb (A25832, dilution 1:25) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x. |
 | Confocal imaging of Jurkat cells using ABflo® 647 Rabbit anti-Human CD99 mAb (A25832, dilution 1:25) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x. |
 | Confocal imaging of MOLT-4 cells using ABflo® 647 Rabbit anti-Human CD99 mAb (A25832, dilution 1:25) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x. |
 | Flow cytometry: 1×10^6 U266 cells (negative control,left) and Jurkat cells (right) were surface-stained with ABflo® 647 Rabbit anti-Human CD99 mAb (A25832,5 μl/Test,orange line) or ABflo® 647 Rabbit IgG isotype control (A22070,5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line). |
 | Flow cytometry: 1×10^6 Jurkat cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070,5 μl/Test,left) or ABflo® 647 Rabbit anti-Human CD99 mAb (A25832,5 μl/Test,right). |
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