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ABflo® 647 Rabbit anti-Human Megalin/LRP2 mAb (100 T)
| Reactivity: | Human |
| Applications: | IF/ICC, FC (intra) |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal Antibody |
| Conjugation: | ABflo® 647. Ex:648nm. Em:664nm. |
| Gene Name: | LDL receptor related protein 2 |
| Gene Symbol: | LRP2 |
| Synonyms: | DBS; GP330; LRP-2 |
| Gene ID: | 4036 |
| UniProt ID: | P98164 |
| Clone ID: | 0C8L4 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 4446-4655 of human Megalin/LRP2 (NP_004516.2). |
| Dilution: | IF/ICC,1:50 - 1:200|FC (intra),5μl per 10^6 cells in 100 μl volume. |
| Purification Method: | Affinity purification |
| Concentration: | 0.04 mg/mL |
| Buffer: | PBS with 0.03% proclin300,0.2% BSA, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-LRP2 Antibody |
Background
The protein encoded by this gene, low density lipoprotein-related protein 2 (LRP2) or megalin, is a multi-ligand endocytic receptor that is expressed in many different tissues but primarily in absorptive epithilial tissues such as the kidney. This glycoprotein has a large amino-terminal extracellular domain, a single transmembrane domain, and a short carboxy-terminal cytoplasmic tail. The extracellular ligand-binding-domains bind diverse macromolecules including albumin, apolipoproteins B and E, and lipoprotein lipase. The LRP2 protein is critical for the reuptake of numerous ligands, including lipoproteins, sterols, vitamin-binding proteins, and hormones. This protein also has a role in cell-signaling; extracellular ligands include parathyroid horomones and the morphogen sonic hedgehog while cytosolic ligands include MAP kinase scaffold proteins and JNK interacting proteins. Recycling of this membrane receptor is regulated by phosphorylation of its cytoplasmic domain. Mutations in this gene cause Donnai-Barrow syndrome (DBS) and facio-oculoacoustico-renal syndrome (FOAR).
Images
 | Confocal imaging of paraffin-embedded Mouse kidney tissue using ABflo® 647 Rabbit anti-Human Megalin/LRP2 mAb (A24716, dilution 1:100). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x. |
 | Confocal imaging of paraffin-embedded Rat kidney tissue using ABflo® 647 Rabbit anti-Human Megalin/LRP2 mAb (A24716, dilution 1:100). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x. |
 | Flow cytometry: 1×10^6 Reh cells (Low Expression,left) and Caco-2 (right) cells were intracellularly-stained with ABflo® 647 Rabbit anti-Human Megalin/LRP2 mAb(A24716,5 μl/Test,orange line) or ABflo® 647 Rabbit IgG isotype control (A22070,5 μl/Test,blue line). Non-fluorescently stained cells was used as blank control (red line). |
 | Flow cytometry: 1×10^6 Caco-2 cells were intracellularly-stained with ABflo® 647 Rabbit IgG isotype control (A22070,5 μl/Test,left) or ABflo® 647 Rabbit anti-Human Megalin/LRP2 mAb (A24716,5 μl/Test,right). |
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