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ABflo® 647 Rabbit anti-Mouse CD69 mAb (100 T)
| Reactivity: | Mouse |
| Applications: | FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal Antibody |
| Conjugation: | ABflo® 647. Ex:648nm. Em:664nm. |
| Gene Name: | CD69 antigen |
| Gene Symbol: | Cd69 |
| Synonyms: | AIM; VEA; 5830438K24Rik |
| Gene ID: | 12515 |
| UniProt ID: | P37217 |
| Clone ID: | 4A0X2 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to aminoacids 64-199 of mouse CD69(NP_001028294.1). |
| Dilution: | FC,5 μl per 10^6 cells in 100 μl volume |
| Purification Method: | Affinity purification |
| Concentration: | 0.04 mg/mL |
| Buffer: | PBS with 0.03% proclin300,0.2% BSA, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-Cd69 antibody |
Background
Predicted to enable calcium ion binding activity and identical protein binding activity. Located in external side of plasma membrane. Orthologous to human CD69 (CD69 molecule).
Images
 | Flow cytometry: 1×10^6 NIH/3T3 cells (negative control,left) and CTLL-2 cells (right) were surface-stained with ABflo® 647 Rabbit anti-Mouse CD69 mAb (A24939,5 μl/Test,orange line) or ABflo® 647 Rabbit IgG isotype control (A22070,5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line). |
 | Flow cytometry: 1×10^6 CTLL-2 cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070,5 μl/Test,left) or ABflo® 647 Rabbit anti-Mouse CD69 mAb (A24939,5 μl/Test,right). |
 | Flow cytometry: 1×10^6 BALB/c mouse splenocytes cells (negative control,left) and BALB/c mouse splenocytes cells (treated with 10 ng/mL PMA for 5 hours,right) were surface-stained with ABflo® 647 Rabbit anti-Mouse CD69 mAb (A24939,5 μl/Test,orange line) or ABflo® 647 Rabbit IgG isotype control (A22070,5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line). |
 | Flow cytometry: 1×10^6 BALB/c mouse splenocytes cells (treated with 10 ng/mL PMA for 5 hours) were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070,5 μl/Test,left) or ABflo® 647 Rabbit anti-Mouse CD69 mAb (A24939,5 μl/Test,right). |
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