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ABflo® 647 Rabbit anti-Mouse IgG1 mAb (100 T)
| Reactivity: | Mouse |
| Applications: | FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal Antibody |
| Conjugation: | ABflo® 647. Ex:648nm. Em:664nm. |
| Gene Symbol: | Ighg1 |
| Synonyms: | IgG1 |
| UniProt ID: | P01868 |
| Clone ID: | 1U2U7 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 98-324 of mouse IgG1(P01868). |
| Dilution: | FC,5 μl per 10^6 cells in 100 μl volume |
| Purification Method: | Affinity purification |
| Concentration: | 0.01 mg/mL |
| Buffer: | PBS with 0.03% proclin300,0.2% BSA, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-Ighg1 antibody |
Background
Mouse IgG1 is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen. It is the most abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response. Mouse IgG1 is composed of two heavy chains and two light chains, and it is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity. It is also involved in the regulation of immune responses, including the activation of T cells and the inhibition of B cell activation. Mouse IgG1 is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry. It is also used in therapeutic applications, such as immunotherapy and vaccine development.
Images
 | Flow cytometry: 1×10^6 PC-3 cells (negative control,left) and THP-1 cells (right) were surface-stained with Mouse anti-Human CD4 mAb ( IgG1, κ,orange line) or ABflo® 647 Mouse IgG1 isotype control (A25488,5 μl/Test,blue line), followed by ABflo® 647 Rabbit anti-Mouse IgG1 mAb (A24925,5 μl/Test) staining. Non-fluorescently stained cells were used as blank control (red line). |
 | Flow cytometry: 1×10^6 THP-1 cells were surface-stained with ABflo® 647 Mouse IgG1 isotype control (A25488,5 μl/Test,left) or Mouse anti-human CD4 mAb (IgG1, κ,right). ABflo® 647 Rabbit anti-Mouse IgG1 mAb (A24925,5 μl/Test) was used as a secondary antibody. |
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