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ABflo® 647 Rabbit anti-Mouse/Rat CD27 mAb (100 T)
| Reactivity: | Mouse |
| Applications: | FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal Antibody |
| Conjugation: | ABflo® 647. Ex:648nm. Em:664nm. |
| Gene Name: | CD27 antigen |
| Gene Symbol: | Cd27 |
| Synonyms: | S152; Tp55; Tnfrsf7 |
| Gene ID: | 21940 |
| UniProt ID: | P41272 |
| Clone ID: | 10U0P4 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 24-182 of mouse CD27 (NP_001028298.1). |
| Dilution: | FC,5 μl per 10^6 cells in 100 μl volume |
| Purification Method: | Affinity purification |
| Concentration: | 0.02 mg/mL |
| Buffer: | PBS with 0.03% proclin300,0.2% BSA, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-Cd27 antibody |
Background
Predicted to enable cysteine-type endopeptidase inhibitor activity involved in apoptotic process. Involved in negative regulation of apoptotic process and positive regulation of JNK cascade. Acts upstream of or within negative regulation of T cell apoptotic process. Located in external side of plasma membrane. Is expressed in several structures, including femur; hemolymphoid system; intestine; pituitary gland; and reproductive system. Human ortholog(s) of this gene implicated in lymphoproliferative syndrome 2. Orthologous to human CD27 (CD27 molecule).
Images
 | Flow cytometry: 1×10^6 A20 cells (negative control,left) and C57BL/6 mouse Splenocytes (right) were surface-stained with ABflo® 647 Rabbit anti-Mouse/Rat CD27 mAb (A25451,5 μl/Test,orange line) or ABflo® 647 Rabbit IgG isotype control (A22070,5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line). |
 | Flow cytometry: 1×10^6 C57BL/6 mouse Splenocytes were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070,5 μl/Test,left) or ABflo® 647 Rabbit anti-Mouse/Rat CD27 mAb (A25451,5 μl/Test,right). |
 | Flow cytometry: 1×10^6 Rat Splenocytes were surface-stained with ABflo® 647 Rabbit anti-Mouse/Rat CD27 mAb (A25451,5 μl/Test,orange line) or ABflo® 647 Rabbit IgG isotype control (A22070,5 μl/Test,blue line). Non-fluorescently stained Rat Splenocytes were used as blank control (red line). |
 | Flow cytometry: 1×10^6 Rat Splenocytes were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070,5 μl/Test,left) or ABflo® 647 Rabbit anti-Mouse/Rat CD27 mAb (A25451,5 μl/Test,right). |
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