ABflo® 647 Rabbit anti-Mouse VISTA PolymAb® (100 T)

Brand: Abclonal
Cat. #: A25974PM
Availability: In Stock

$308.00

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Description

Reactivity:	Mouse
Applications:	FC
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Monoclonal Antibody
Conjugation:	ABflo® 647. Ex:648nm. Em:664nm.
Gene Name:	V-set immunoregulatory receptor
Gene Symbol:	Vsir
Synonyms:	Dies1; PD-1H; VISTA; 4632428N05Rik
Gene ID:	74048
UniProt ID:	Q9D659
Clone ID:	2D5A4
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 33-191 of mouse VISTA (NP_083008.1).
Dilution:	FC,5 μl per 10^6 cells in 100 μl volume
Purification Method:	Affinity purification
Concentration:	0.5mg/ml
Buffer:	PBS with 0.03% proclin300,0.2% BSA, pH7.3.
Storage:	Store at -20°C. Avoid freeze / thaw cycles.
Documents:	Manual-Vsir antibody

Background

Predicted to enable endopeptidase activator activity; enzyme binding activity; and identical protein binding activity. Acts upstream of or within several processes, including negative regulation of CD4-positive, alpha-beta T cell proliferation; negative regulation of T cell cytokine production; and positive regulation of BMP signaling pathway. Located in external side of plasma membrane. Is expressed in several structures, including heart; immune system; lung; male reproductive gland or organ; and small intestine lamina propria. Orthologous to human VSIR (V-set immunoregulatory receptor).

Images

	Flow cytometry: 1×10^6 NIH/3T3 cells (negative control,left) and C57BL/6 mouse splenocytes (right) were surface-stained with ABflo® 647 Rabbit anti-Mouse VISTA PolymAb® (A25974PM,5 μl/Test,orange line) or ABflo® 647 Rabbit IgG isotype control (A22070,5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line).
	Flow cytometry: 1×10^6 C57BL/6 mouse splenocytes were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070,5 μl/Test,left) or ABflo® 647 Rabbit anti-Mouse VISTA PolymAb® (A25974PM,5 μl/Test,right).