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ABflo® 647 Rabbit anti-puromycin mAb (100 T)
| Applications: | FC (intra) |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal Antibody |
| Conjugation: | ABflo® 647. Ex:648nm. Em:664nm. |
| Gene Symbol: | Puromycin |
| Synonyms: | Puromycin |
| Gene ID: | CAS:58-58-2 |
| Clone ID: | 9D5C3 |
| Immunogen: | Chemical compounds corresponding to puromycin. |
| Dilution: | FC (intra),5 μl per 10^6 cells in 100 μl volume |
| Purification Method: | Affinity purification |
| Concentration: | 0.04 mg/mL |
| Buffer: | PBS with 0.03% proclin300,0.2% BSA, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-Puromycin antibody |
Background
Puromycin is an aminonucleoside antibiotic, derived from the Streptomyces alboniger bacterium, that causes premature chain termination during translation taking place in the ribosome. It has a role as a nucleoside antibiotic, an antiinfective agent, an antineoplastic agent, a protein synthesis inhibitor, an antimicrobial agent, an EC 3.4.11.14 (cytosol alanyl aminopeptidase) inhibitor and an EC 3.4.14.2 (dipeptidyl-peptidase II) inhibitor. It is a conjugate base of a puromycin(1+).Puromycin is an antibiotic that prevents bacterial protein translation. It is utilized as a selective agent in laboratory cell cultures. Puromycin is toxic to both prokaryotic and eukaryotic cells, resulting in significant cell death at appropriate doses.
Images
 | Flow cytometry:1×10^6 293T cells (negative control,Left) and 293T (treated with puromycin,right) cells were intracellularly-stained with ABflo® 647 Rabbit anti-puromycin mAb(A23131,5 μl/Test,orange line) or ABflo® 647 Rabbit IgG isotype control (A22070,5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line). |
 | Flow cytometry:1×10^6 293T cells(treated with puromycin) were intracellularly-stained with ABflo® 647 Rabbit IgG isotype control (A22070,5 μl/Test,left) or ABflo® 647 Rabbit anti-puromycin mAb(A23131,5 μl/Test,right). |
 | Flow cytometry:1×10^6 RAW 264.7 cells (negative control,Left) and RAW 264.7 (treated with puromycin,right) cells were intracellularly-stained with ABflo® 647 Rabbit anti-puromycin mAb(A23131,5 μl/Test,orange line) or ABflo® 647 Rabbit IgG isotype control (A22070,5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line). |
 | Flow cytometry:1×10^6 Raw264.7 cells (treated with puromycin) were intracellularly-stained with ABflo® 647 Rabbit IgG isotype control (A22070,5 μl/Test,left) or ABflo® 647 Rabbit anti-puromycin mAb(A23131,5 μl/Test,right). |
 | Flow cytometry:1×10^6 C6 cells (negative control,Left) and C6 (treated with puromycin,right) cells were intracellularly-stained with ABflo® 647 Rabbit anti-puromycin mAb(A23131,5 μl/Test,orange line) or ABflo® 647 Rabbit IgG isotype control (A22070,5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line). |
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