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APC Rabbit anti-Mouse CD32 mAb (100 T)
| Reactivity: | Mouse |
| Applications: | FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal Antibody |
| Conjugation: | APC. Ex:650nm. Em:660nm. |
| Gene Name: | Fc receptor, IgG, low affinity IIb |
| Gene Symbol: | Fcgr2b |
| Synonyms: | CD32; Fcgr2; Fcr-2; Fcr-3; Ly-17; LyM-1; Lym-1; fcRII; FcgRII; Fcgr2a; Ly-m20; Fc[g]RII; F630109E10Rik |
| Gene ID: | 14130 |
| UniProt ID: | P08101 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 30-207 of mouse CD32 (NP_034317.1). |
| Dilution: | FC,5 μl per 10^6 cells in 100 μl volume |
| Purification Method: | Affinity purification |
| Concentration: | 0.04 mg/ml |
| Buffer: | PBS with 0.09% Sodium azide,0.2% BSA, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-Fcgr2b antibody |
Background
CD32 (Fcgr2) is a 40 kD transmembrane glycoprotein, member of the immunoglobulin superfamily. The extracellular region of CD32 consists of two Ig C-type domains that binds the Fc region from monomeric IgG with low affinity, but binds immune complexes efficiently. CD32 can mediate phagocytosis of immune complexes and modulate cell activation. CD32 is expressed by Macrophages, neutrophils, mast cells and B cells.
Images
 | Flow cytometry: 1×10^6 bEnd.3 cells (negative control,left) and C57BL/6 mouse splenocytes (right) were surface-stained with APC Rabbit anti-Mouse CD32 mAb (A26143,5 μl/Test,orange line) or APC Rabbit IgG isotype control (A24173,5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line). |
 | Flow cytometry: 1×10^6 C57BL/6 mouse splenocytes were surface-stained with APC Rabbit IgG isotype control (A24173,5 μl/Test,left) or APC Rabbit anti-Mouse CD32 mAb (A26143,5 μl/Test,right). |
 | Flow cytometry:1×10^6 C57BL/6 mouse splenocytes were surface-stained with PE Rabbit anti-Mouse CD19 mAb (A25265,5 μl/Test) and APC Rabbit anti-Mouse CD32 mAb (A26143,5 μl/Test,right) or APC Rabbit IgG isotype control (A24173,5 μl/Test,left). |
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