PE/Cyanine7 Rabbit anti-Human IFN-gamma mAb (100 T)

Background

This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases.

Images

	Flow cytometry: 1×10^6 293F cells (negative control,left) and 293F (Transfection,right) cells were intracellularly-stained with PE/Cyanine7 Rabbit anti-Human IFN-gamma mAb (A27517,5 μl/Test,orange line) or PE/Cyanine7 Rabbit IgG isotype control (5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line).
	Flow cytometry: 1×10^6 293F (Transfection) cells were intracellularly-stained with PE/Cyanine7 Rabbit IgG isotype control (5 μl/Test,left) or PE/Cyanine7 Rabbit anti-Human IFN-gamma mAb (A27517,5 μl/Test,right).
	Flow cytometry: 1×10^6 Human PBMC (untreated,left) andHuman PBMC (treated with 50 ng/mL PMA and 1 ug/mL Ionomycin for 4-6 hours in the presence of monensin,right) were intracellularly-stained with PE/Cyanine7 Rabbit anti-Human IFN-gamma mAb (A27517,5 μl/Test,orange line) or PE/Cyanine7 Rabbit IgG isotype control (5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line).
	Flow cytometry: 1×10^6 Human PBMC (untreated,left) and Human PBMC (treated with 50 ng/mL PMA and 1 ug/mL Ionomycin for 4-6 hours in the presence of monensin,right) were intracellularly-stained with PE/Cyanine7 Rabbit anti-Human IFN-gamma mAb (A27517,5 μl/Test).

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