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PE/Cyanine7 Rabbit anti-Mouse CD4 mAb (100 T)
| Reactivity: | Mouse |
| Applications: | FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal Antibody |
| Conjugation: | PE-Cy7. Ex:565nm. Em:778nm. |
| Gene Name: | CD4 antigen |
| Gene Symbol: | Cd4 |
| Synonyms: | L3T4; Ly-4 |
| Gene ID: | 12504 |
| UniProt ID: | P06332 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 27-394 of mouse CD4 (NP_038516.1). |
| Dilution: | FC,5 μl per 10^6 cells in 100 μl volume |
| Purification Method: | Affinity purification |
| Concentration: | 0.04mg/ml |
| Buffer: | PBS with 0.09% Sodium azide,0.2% BSA, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-Cd4 Antibody |
Background
Predicted to enable several functions, including interleukin-16 binding activity; interleukin-16 receptor activity; and protein homodimerization activity. Involved in helper T cell enhancement of adaptive immune response. Acts upstream of or within several processes, including defense response to Gram-negative bacterium; positive regulation of calcium-mediated signaling; and positive regulation of peptidyl-tyrosine phosphorylation. Located in endoplasmic reticulum; external side of plasma membrane; and membrane raft. Is expressed in several structures, including alimentary system; brain; genitourinary system; hemolymphoid system; and liver and biliary system. Used to study type 1 diabetes mellitus. Human ortholog(s) of this gene implicated in immunodeficiency 79. Orthologous to human CD4 (CD4 molecule).
Images
 | Flow cytometry: 1×10^6 C57BL/6 mouse splenocytes were surface-stained with PE/Cyanine7 Rabbit anti-Mouse CD4 mAb (A27150,5 μl/Test,orange line) or PE/Cyanine7 Rabbit IgG isotype control (5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line). |
 | Flow cytometry: 1×10^6 293T cells (negative control,left) and 293T (Transfection,right) cells were surface-stained with PE/Cyanine7 Rabbit anti-Mouse CD4 mAb (A27150,5 μl/Test,orange line) or PE/Cyanine7 Rabbit IgG isotype control (5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line). |
 | Flow cytometry: 1×10^6 293T cells (negative control,left) and 293T (Transfection,right) cells were surface-stained with PE/Cyanine7 Rabbit anti-Mouse CD4 mAb (A27150,5 μl/Test,orange line) or PE/Cyanine7 Rabbit IgG isotype control (5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line). |
 | Flow cytometry: 1×10^6 C57BL/6 mouse splenocytes were surface-stained with PE/Cyanine7 Rabbit IgG isotype control (5 μl/Test,left) or PE/Cyanine7 Rabbit anti-Mouse CD4 mAb (A27150,5 μl/Test,right). |
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