| Reactivity: | Human |
| Applications: | FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal Antibody |
| Conjugation: | PE. Ex:565nm. Em:574nm. |
| Gene Name: | 5'-nucleotidase ecto |
| Gene Symbol: | NT5E |
| Synonyms: | NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA |
| Gene ID: | 4907 |
| UniProt ID: | P21589 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 27-547 of human NT5E/CD73 (NP_002517.1). |
| Dilution: | FC,5 μl per 10^6 cells in 100 μl volume |
| Purification Method: | Affinity purification |
| Concentration: | 0.02 mg/ml |
| Buffer: | PBS with 0.09% Sodium azide,0.2% BSA, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-NT5E Antibody |
Background
The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.
Images
![]() | Flow cytometry: 1×10^6 MOLT-4 cells (negative control,left) and A-431 cells (right) were surface-stained with PE Rabbit anti-Human NT5E/CD73 mAb (A26651,5 μl/Test,orange line) or PE Rabbit IgG isotype control (A24172,5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line). |
![]() | Flow cytometry: 1×10^6 Human PBMC were surface-stained with PE Rabbit anti-Human NT5E/CD73 mAb (A26651,5 μl/Test,orange line) or PE Rabbit IgG isotype control (A24172,5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line). |
![]() | Flow cytometry: 1×10^6 A-431 cells were surface-stained with PE Rabbit IgG isotype control (A24172,5 μl/Test,left) or PE Rabbit anti-Human NT5E/CD73 mAb (A26651,5 μl/Test,right). |
![]() | Flow cytometry: 1×10^6 Human PBMC were surface-stained with PE Rabbit IgG isotype control (A24172,5 μl/Test,left) or PE Rabbit anti-Human NT5E/CD73 mAb (A26651,5 μl/Test,right).Cells in the Lymphocytes gate were used for analysis. |
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