Your shopping cart is empty!
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC, IF/IC, IP, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | Growth differentiation factor 15 |
| Gene Symbol: | GDF15 |
| Synonyms: | PDF; MIC1; PLAB; MIC-1; NAG-1; PTGFB; GDF-15; GDF15 |
| Gene ID: | 9518 |
| UniProt ID: | Q99988 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 49-308 of human GDF15 (NP_004855.2). |
| Dilution: | WB 1:500-1:1000; IHC 1:50-1:200; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-GDF15 antibody |
Background
This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. The protein is expressed in a broad range of cell types, acts as a pleiotropic cytokine and is involved in the stress response program of cells after cellular injury. Increased protein levels are associated with disease states such as tissue hypoxia, inflammation, acute injury and oxidative stress.
Images
 | Western blot analysis of various lysates using GDF15 Rabbit pAb (A0185) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunohistochemistry analysis of paraffin-embedded Human placenta using GDF15 Rabbit pAb (A0185) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining. |
 | Immunofluorescence analysis of BALB-3T3 cells using GDF15 Rabbit pAb (A0185) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
 | Immunoprecipitation analysis of 200 μg extracts of HT-1080 cells using 3 μg GDF15 antibody (A0185). Western blot was performed from the immunoprecipitate using GDF15 antibody (A0185) at a dilution of 1:1000. |
You may also be interested in:
Related Products
-10%
-16%
