Anti-GFAP Rabbit pAb (100 μl)

Anti-GFAP Rabbit pAb (100 μl)

Brand: ServiceBio
Cat. #: GB11096-100
Availability: In Stock
$259.00
-+
Reactivity:  Human, Mouse, Rat    
Applications:  WB, IHC/IF    
Host Species:  Rabbit    
Clonality:  Polyclonal    
Gene Name: Glial fibrillary acidic protein
Gene Symbol: GFAP
Synonyms: ALXDRD
Gene ID: 2670
Uniprot ID: P14136, P03995, P47819
Immunogen: KLH conjugated Synthetic peptide corresponding to Mouse GFAP
Isotype: IgG
Purity: Affinity purification
Subcellular location: Cytoplasm
Documents: Manual-GFAP Antibody




Product Usage Information

WB

Human, Mouse, Rat

1: 1000-1: 2000

brain, cerebellum, cortex

IHC/IF

Human, Mouse, Rat

1: 500-1: 3000

brain, cerebellum




Background

The gene GFAP encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms.




Images

gfap wb

WB analysis of GFAP (GB11096).

Sample: Protein treated by RIPA Lysis Buffer (G2002).

Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.

Primary antibody: 1: 2000, 4°C overnight.

Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 3000, RT, 1h.

anti GFAP antibody

IHC analysis of GFAP (GB11096).

Sample: Rat brain (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98°C,20 min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 3000, 4°C overnight.

Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.

anti gfap

IHC analysis of GFAP (GB11096).

Sample: Mouse brain (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98°C,20 min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 3000, 4°C overnight.

Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.

gfap immunohistochemistry

IHC analysis of GFAP (GB11096).

Sample: Rat cerebellum (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98°C,20 min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 2800, 4°C overnight.

Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.

GFAP antibody

IF analysis of GFAP (GB11096).

Sample: Mouse brain (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98°C,20 min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 2000, 4°C overnight.

Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h.

rabbit anti gfap

IF analysis of GFAP (GB11096).

Sample: Rat brain (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98°C,20 min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 2000, 4°C overnight.

Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h.




Storage

Storage Store at -20 °C for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.



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