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Anti-HIP2/LIG Rabbit pAb (100 μl)
| Reactivity: | H,M,R & H |
| Applications: | WB & IHC |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | HIP2/LIG rabbit polyclonal |
Gene Name: | Ubiquitin-conjugating enzyme E2 K |
Synonyms: | E2-25K, HIP 2, HIP2, HYPG, LIG, UBE2K, Ubiquitin carrier protein, Ubiquitin protein ligase |
Immunogen: | Recombinant protein corresponding to Mouse HIP2/LIG |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | |
Predicted MW. | 22 kDa |
Observed MW. | 26 kDa |
Uniprot ID: |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Human, Mouse, Rat | 1: 300-1: 1000 | spleen, thymus, lymph node, colon |
IHC | Human | 1: 1000-1: 2000 | colon cancer, colon, kidney, placenta |
Background
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro, in the presence or in the absence of BRCA1-BARD1 E3 ubiquitin-protein ligase complex, catalyzes the synthesis of 'Lys-48'-linked polyubiquitin chains. Does not transfer ubiquitin directly to but elongates monoubiquitinated substrate protein. Mediates the selective degradation of short-lived and abnormal proteins, such as the endoplasmic reticulum-associated degradation (ERAD) of misfolded lumenal proteins.
Images
| Western blot analysis of LIG (GB113541) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin-embedded human colon cancer using LIG (GB113541) at dilution of 1: 2000 |
| Immunohistochemistry analysis of paraffin-embedded human colon using LIG (GB113541) at dilution of 1: 2000 |
| Immunohistochemistry analysis of paraffin-embedded human kidney using LIG (GB113541) at dilution of 1: 2000 |
| Immunohistochemistry analysis of paraffin-embedded human placenta using LIG (GB113541) at dilution of 1: 2000 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |