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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | major histocompatibility complex, class II, DP beta 1 |
| Gene Symbol: | HLA-DPB1 |
| Synonyms: | DPB1; HLA-DP; HLA-DPB; HLA-DP1B; HLA-DPB1 |
| Gene ID: | 3115 |
| UniProt ID: | P04440 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 30-225 of human HLA-DPB1 (NP_002112.3). |
| Dilution: | WB 1:500-1:1000; IHC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 1.02mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-HLA-DPB1 antibody |
Background
HLA-DPB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DPA) and a beta chain (DPB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DP molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to 4 different molecules.
Images
 | Western blot analysis of various lysates, using HLA-DPB1 Rabbit pAb (A1412) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Negative control (NC): HeLa Exposure time: 10s. |
 | Immunohistochemistry analysis of paraffin-embedded Human lymphonodus using HLA-DPB1 Rabbit pAb (A1412) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human stomach using HLA-DPB1 Rabbit pAb (A1412) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Rat ovary using HLA-DPB1 Rabbit pAb (A1412) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human lung cancer using HLA-DPB1 Rabbit pAb (A1412) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
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