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| Reactivity: | Human |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | major histocompatibility complex, class II, DR beta 4 |
| Gene Symbol: | HLA-DRB4 |
| Synonyms: | DR4; DRB4; HLA-DRB; HLA-DR4B; HLA-DRB4 |
| Gene ID: | 3126 |
| UniProt ID: | P13762 |
| Clone ID: | 3Z5V9 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 50-150 of human HLA-DRB4 (P13762). |
| Dilution: | WB 1:1000-1:6000 |
| Purification Method: | Affinity purification |
| Concentration: | 0.40 mg/mL |
| Buffer: | PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-HLA-DRB4 antibody |
Background
HLA-DRB4 belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DRA) and a beta (DRB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells. The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. There are multiple pseudogenes of this gene.
Images
 | Western blot analysis of lysates from Raji cells, using HLA-DRB4 Rabbit mAb (A0439) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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