Your shopping cart is empty!
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC, IF/IC, IP, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | heterogeneous nuclear ribonucleoprotein A1 |
| Gene Symbol: | HNRNPA1 |
| Synonyms: | UP 1; ALS19; ALS20; HNRPA1; IBMPFD3; HNRPA1L3; hnRNP A1; hnRNP-A1 |
| Gene ID: | 3178 |
| UniProt ID: | P09651 |
| Clone ID: | 10N0F4 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 250-350 of human hnRNP A1 (P09651). |
| Dilution: | WB 1:1000-1:2000; IHC 1:200-1:2000; IF/IC 1:200-1:2000 |
| Purification Method: | Affinity purification |
| Concentration: | 1.30 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-HNRNPA1 antibody |
Background
This gene encodes a member of a family of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs), which are RNA-binding proteins that associate with pre-mRNAs in the nucleus and influence pre-mRNA processing, as well as other aspects of mRNA metabolism and transport. The protein encoded by this gene is one of the most abundant core proteins of hnRNP complexes and plays a key role in the regulation of alternative splicing. Mutations in this gene have been observed in individuals with amyotrophic lateral sclerosis 20. Multiple alternatively spliced transcript variants have been found. There are numerous pseudogenes of this gene distributed throughout the genome.hnRNP A1 has three isoforms with MW 30 kDa, 34 kDa and 39 kDa.
Images
 | Western blot analysis of various lysates using hnRNP A1 Rabbit mAb (A11564) at 1：1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Western blot analysis of various lysates using hnRNP A1 Rabbit mAb (A11564) at 1：1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min. |
 | Immunohistochemistry analysis of paraffin-embedded Human colon using hnRNP A1 Rabbit mAb (A11564) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
 | Confocal imaging of U-2 OS cells using hnRNP A1 Rabbit mAb (A11564,dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007,dilution 1:500)(Red).The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green).DAPI was used for nuclear staining (Blue). Objective: 100x. |
 | Confocal imaging of NIH/3T3 cells using hnRNP A1 Rabbit mAb (A11564,dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007,dilution 1:500)(Red).The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green).DAPI was used for nuclear staining (Blue). Objective: 100x. |
You may also be interested in:
