ICMT Rabbit pAb (20 μl)

ICMT Rabbit pAb (20 μl)

Brand: Abclonal
Cat. #: A10293
Availability: In Stock
$108.00
-+
Reactivity: Human, Mouse, Rat    
Applications: WB, IHC, ELISA    
Host Species: Rabbit    
Clonality: Polyclonal antibody    
Gene Name: isoprenylcysteine carboxyl methyltransferase
Gene Symbol: ICMT
Synonyms: PCMT; PPMT; PCCMT; HSTE14; MST098; MSTP098; ICMT
Gene ID: 23463
UniProt ID: O60725
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 175-284 of human ICMT (NP_036537.1).
Dilution: WB 1:500-1:2000; IHC 1:50-1:200
Purification Method: Affinity purification
Concentration: 1.34 mg/ml
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at -20°C. Avoid freeze / thaw cycles.
Documents: Manual-ICMT antibody




Background

This gene encodes the third of three enzymes that posttranslationally modify isoprenylated C-terminal cysteine residues in certain proteins and target those proteins to the cell membrane. This enzyme localizes to the endoplasmic reticulum. Alternative splicing may result in other transcript variants, but the biological validity of those transcripts has not been determined.




Images

ICMT antibodyWestern blot analysis of various lysates using ICMT Rabbit pAb (A10293) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Rat heart using ICMT Rabbit pAb (A10293) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat testis using ICMT Rabbit pAb (A10293) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.



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