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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF/IC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | interleukin 1 receptor associated kinase 1 |
| Gene Symbol: | IRAK1 |
| Synonyms: | IRAK; pelle; IRAK1 |
| Gene ID: | 3654 |
| UniProt ID: | P51617 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 424-633 of human IRAK1 (NP_001020414.1). |
| Dilution: | WB 1:1000-1:3000; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 0.59 mg/ml |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-IRAK1 antibody |
Background
This gene encodes the interleukin-1 receptor-associated kinase 1, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. This gene is partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Images
 | Western blot analysis of lysates from 293T cells, using IRAK1 Rabbit pAb (A12624) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunofluorescence analysis of C6 cells using IRAK1 Rabbit pAb (A12624) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of HeLa cells using IRAK1 Rabbit pAb (A12624) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of NIH/3T3 cells using IRAK1 Rabbit pAb (A12624) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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