Reactivity: | Human |
Applications: | WB, IF/IC, ELISA |
Host Species: | Rabbit |
Isotype: | IgG |
Clonality: | Polyclonal antibody |
Gene Name: | Janus kinase 3 |
Gene Symbol: | JAK3 |
Synonyms: | JAKL; LJAK; JAK-3; L-JAK; JAK3_HUMAN; JAK3 |
Gene ID: | 3718 |
UniProt ID: | P52333 |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 350-530 of JAK3 (NP_000206.2). |
Dilution: | WB 1:1000-1:5000; IF/IC 1:50-1:200 |
Purification Method: | Affinity purification |
Concentration: | 2.13 mg/mL |
Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
Documents: | Manual-JAK3 antibody |
Background
The protein encoded by this gene is a member of the Janus kinase (JAK) family of tyrosine kinases involved in cytokine receptor-mediated intracellular signal transduction. It is predominantly expressed in immune cells and transduces a signal in response to its activation via tyrosine phosphorylation by interleukin receptors. Mutations in this gene are associated with autosomal SCID (severe combined immunodeficiency disease).
Images
![]() | Western blot analysis of various lysates, using JAK3 Rabbit pAb (A0748) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s. |
![]() | Immunofluorescence analysis of TF-1 cells using JAK3 Rabbit pAb (A0748) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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