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| Reactivity: | Human |
| Applications: | WB, IHC, IF/IC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | adenosine deaminase RNA specific |
| Gene Symbol: | ADAR |
| Synonyms: | DSH; AGS6; G1P1; IFI4; P136; ADAR1; DRADA; DSRAD; IFI-4; K88DSRBP |
| Gene ID: | 103 |
| UniProt ID: | P55265 |
| Clone ID: | 8T1W3 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 610-810 of human ADAR11 (NP_001102.3). |
| Dilution: | WB 1:5000-1:30000; IHC 1:200-1:2000; IF/IC 1:100-1:400 |
| Purification Method: | Affinity purification |
| Concentration: | 1.59 mg/mL |
| Buffer: | PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-ADAR monoclonal antibody |
Background
The gene ADAR encodes the enzyme responsible for RNA editing by site-specific deamination of adenosines. This enzyme destabilizes double-stranded RNA through conversion of adenosine to inosine. Mutations in this gene have been associated with dyschromatosis symmetrica hereditaria. Alternative splicing results in multiple transcript variants.
Images
 | Western blot analysis of lysates from wild type (WT) and ADAR1 knockdown (KD) HeLa cells using [KD Validated] ADAR1 Rabbit mAb (A27294) at 1:5000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using [KD Validated] ADAR1 Rabbit mAb (A27294) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using [KD Validated] ADAR1 Rabbit mAb (A27294) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human colon tissue using [KD Validated] ADAR1 Rabbit mAb (A27294) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using [KD Validated] ADAR1 Rabbit mAb (A27294) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining. |
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