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| Reactivity: | Human, Rat |
| Applications: | WB, FC |
| Host Species: | Mouse |
| Isotype: | IgG |
| Clonality: | Oligoclonal antibody |
| Gene Name: | adducin 1 |
| Gene Symbol: | ADD1 |
| Synonyms: | ADDA |
| Gene ID: | 118 |
| UniProt ID: | P35611 |
| Immunogen: | Recombinant protein of human ADD1 |
| Dilution: | WB 1:500-1:2,500; FC 1:2,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
Adducins are a family of cytoskeletal proteins encoded by three genes (alpha, beta, and gamma). Adducin acts as a heterodimer of the related alpha, beta, or gamma subunits. The protein encoded by this gene represents the alpha subunit. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C.
Images
 | Western blotting analysis using anti-adducin 1 antibody (Cat#65299). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-adducin 1 antibody (Cat#65299, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-adducin 1 antibody (Cat#65299). Adducin 1 expression in wild-type (WT) and adducin 1 (ADD1) shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-adducin 1 antibody (Cat#65299, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Flow cytometric analysis of Adducin 1 expression in HT-1080 cells using anti-Adducin 1 antibody (Cat#65299, 1:2,000). Green, isotype control; red, Adducin 1. |
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