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| Reactivity: | Human, Rat |
| Applications: | WB, FC |
| Host Species: | Mouse |
| Isotype: | IgG1 |
| Clonality: | Monoclonal antibody |
| Gene Name: | adenylosuccinate lyase |
| Gene Symbol: | ADSL |
| Synonyms: | ASL; AMPS; ASASE |
| Gene ID: | 158 |
| UniProt ID: | P30566 |
| Clone ID: | 25GB595 |
| Immunogen: | Recombinant protein of human ADSL |
| Dilution: | WB 1:2,500-1:5,000; FC 1:2,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein encoded by the gene ADSL belongs to the lyase 1 family. It is an essential enzyme involved in purine metabolism, and catalyzes two non-sequential reactions in the de novo purine biosynthetic pathway: the conversion of succinylaminoimidazole carboxamide ribotide (SAICAR) to aminoimidazole carboxamide ribotide (AICAR) and the conversion of adenylosuccinate (S-AMP) to adenosine monophosphate (AMP). Mutations in this gene are associated with adenylosuccinase deficiency (ADSLD), a disorder marked with psychomotor retardation, epilepsy or autistic features. Alternatively spliced transcript variants have been found for this gene.
Images
 | Western blotting analysis using anti-adenylosuccinate lyase antibody (Cat#61256). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-adenylosuccinate lyase antibody (Cat#61256, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-adenylosuccinate lyase antibody (Cat#61256). Adenylosuccinate lyase expression in wild type (WT) and adenylosuccinate lyase (ADSL) knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-adenylosuccinate lyase antibody (Cat#61256, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Flow cytometric analysis of Adenylosuccinate lyase expression in HeLa cells using anti-Adenylosuccinate lyase antibody (Cat# 61256, 1:2,000). Green, isotype control; red, Adenylosuccinate lyase. |
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