Your shopping cart is empty!
| Reactivity: | Human, Mouse |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | argonaute RISC catalytic component 3 |
| Gene Symbol: | AGO3 |
| Synonyms: | EIF2C3 |
| Gene ID: | 192669 |
| UniProt ID: | Q9H9G7 |
| Clone ID: | 23GB3930 |
| Immunogen: | A synthesized peptide derived from human EIF2C3 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, contains a PAZ domain and a PIWI domain, and may play a role in short-interfering-RNA-mediated gene silencing. This gene is located on chromosome 1 in a tandem cluster of closely related family members including argonaute 4 and eukaryotic translation initiation factor 2C, 1. Two transcript variants encoding distinct isoforms have been identified for this gene.
Images
 | Western blotting analysis using anti-AGO3 antibody (Cat#68651). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-AGO3 antibody (Cat#68651, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-AGO3 antibody (Cat#68651). AGO3 expression in wild-type (WT) and AGO3 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-AGO3 antibody (Cat#68651, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of AGO3 expression in HeLa cells using AGO3 antibody (Cat#68651, 1:2,000). Green, isotype control; red, AGO3. |
 | Immunocytochemical staining of Hela cells with AGO3 antibody (Cat#68651, 1:1,000). Nuclei were stained blue with DAPI; AGO3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
You may also be interested in:
