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| Reactivity: | Human |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | alkylglycerone phosphate synthase |
| Gene Symbol: | AGPS |
| Synonyms: | ADAS; ADPS; RCDP3; ADAP-S; ADHAPS; ALDHPSY |
| Gene ID: | 8540 |
| UniProt ID: | O00116 |
| Clone ID: | 25GB3850 |
| Immunogen: | Recombinant protein of human Alkyl-DHAP synthase |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene is a member of the FAD-binding oxidoreductase/transferase type 4 family. It encodes a protein that catalyzes the second step of ether lipid biosynthesis in which acyl-dihydroxyacetonephosphate (DHAP) is converted to alkyl-DHAP by the addition of a long chain alcohol and the removal of a long-chain acid anion. The protein is localized to the inner aspect of the peroxisomal membrane and requires FAD as a cofactor. Mutations in this gene have been associated with rhizomelic chondrodysplasia punctata, type 3 and Zellweger syndrome.
Images
 | Western blotting analysis using anti-alkylglycerone phosphate synthase antibody (Cat#61813). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-alkylglycerone phosphate synthase antibody (Cat#61813, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-alkylglycerone phosphate synthase antibody (Cat#61813). Alkylglycerone phosphate synthase expression in wild-type (WT) and alkylglycerone phosphate synthase (AGPS) shRNA knockdown (KD) HepG2 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-alkylglycerone phosphate synthase antibody (Cat#61813, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of Alkylglycerone phosphate synthase expression in HeLa cells using anti-Alkylglycerone phosphate synthase antibody (Cat#61813, 1:2,000). Green, isotype control; red, Alkylglycerone phosphate synthase. |
 | Immunocytochemical staining of Hela cells with anti-Alkylglycerone phosphate synthase antibody (Cat#61813, 1:1,000). Nuclei were stained blue with DAPI; Alkylglycerone phosphate synthase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm. |
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