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| Reactivity: | Human, Rat |
| Applications: | WB, IP, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | ajuba LIM protein |
| Gene Symbol: | AJUBA |
| Synonyms: | JUB; [KD Validated] Ajuba |
| Gene ID: | 84962 |
| UniProt ID: | Q96IF1 |
| Clone ID: | 8P3P8 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 201-290 of human Ajuba (NP_116265.1). |
| Dilution: | WB 1:2000-1:20000 |
| Purification Method: | Affinity purification |
| Concentration: | 0.5 mg/ml |
| Buffer: | PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-AJUBA monoclonal antibody |
Background
Enables alpha-catenin binding activity and transcription corepressor activity. Involved in several processes, including negative regulation of hippo signaling; positive regulation of gene silencing by miRNA; and regulation of cellular response to hypoxia. Acts upstream of or within gene silencing by miRNA and positive regulation of protein-containing complex assembly. Located in several cellular components, including Golgi apparatus; P-body; and nucleoplasm.
Images
 | Western blot analysis of lysates from Rat lung using [KD Validated] Ajuba Rabbit mAb (A22039) at1:20000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 180s. |
 | Western blot analysis of lysates from wild type (WT) and Ajuba knockdown (KD) HepG2 cells, using [KD Validated] Ajuba Rabbit mAb (A22039) at1:20000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunoprecipitation analysis of 300 μg extracts of HepG2 cells using 3 μg Ajuba antibody (A22039). Western blot was performed from the immunoprecipitate using Ajuba (A22039) at a dilution of 1:10000. |
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