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| Reactivity: | Human |
| Applications: | WB, FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | A-Raf proto-oncogene, serine/threonine kinase |
| Gene Symbol: | ARAF |
| Synonyms: | PKS2; A-RAF; ARAF1; RAFA1 |
| Gene ID: | 369 |
| UniProt ID: | P10398 |
| Clone ID: | 25GB440 |
| Immunogen: | A synthesized peptide derived from human A-RAF |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
Enables protein serine/threonine kinase activity. Involved in negative regulation of apoptotic process; regulation of TOR signaling; and regulation of protein metabolic process. Predicted to be active in cytosol and mitochondrion. Biomarker of high grade glioma.
Images
 | Western blotting analysis using anti-ARAF antibody (Cat#61218). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ARAF antibody (Cat#61218, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-ARAF antibody (Cat#61218). ARAF expression in wild type (WT) and ARAF knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ARAF antibody (Cat#61218, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of ARAF expression in HepG2 cells using anti-ARAF antibody (Cat# 61218, 1:2,000). Green, isotype control; red, ARAF. |
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