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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | BCL2 apoptosis regulator |
| Gene Symbol: | BCL2 |
| Synonyms: | Bcl-2; PPP1R50 |
| Gene ID: | 596 |
| UniProt ID: | P10415 |
| Clone ID: | 23GB675 |
| Immunogen: | A synthesized peptide derived from human Bcl-2 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The gene BCL2 encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants.
Images
 | Western blotting analysis using anti-Bcl 2 antibody (Cat#68142). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Bcl 2 antibody (Cat#68142, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-Bcl-2 antibody (Cat#68142). Bcl-2 expression in wild type (WT) and Bcl-2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Bcl-2 antibody (Cat#68142, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of Bcl-2 expression in HepG2 cells using anti-Bcl-2 antibody (Cat#68142, 1:2,000). Green, isotype control;red, Bcl-2. |
 | Immunocytochemical staining of HepG2 cells with Bcl-2 antibody (Cat#68142, 1:1,000). Nuclei were stained blue with DAPI; Bcl-2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
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