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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | Brain derived neurotrophic factor |
| Gene Symbol: | BDNF |
| Synonyms: | ANON2; BULN2 |
| Gene ID: | 627 |
| UniProt ID: | P23560 |
| Clone ID: | 23GB3005 |
| Immunogen: | A synthesized peptide derived from human BDNF |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The gene BDNF encodes a member of the nerve growth factor family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protein. Binding of this protein to its cognate receptor promotes neuronal survival in the adult brain. Expression of this gene is reduced in Alzheimer's, Parkinson's, and Huntington's disease patients. This gene may play a role in the regulation of the stress response and in the biology of mood disorders.
Images
 | Western blotting analysis using anti-Brain derived neurotrophic factor antibody (Cat#69354). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Brain derived neurotrophic factor antibody (Cat#69354, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-Brain derived neurotrophic factor antibody (Cat#69354). Brain derived neurotrophic factor expression in wild type (WT) and Brain derived neurotrophic factor shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Brain derived neurotrophic factor antibody (Cat#69354, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Flow cytometric analysis of Brain derived neurotrophic factor expression in HepG2 cells using Brain derived neurotrophic factor antibody (Cat#69354, 1:2,000). Green, isotype control; red, Brain derived neurotrophic factor. |
 | Immunocytochemical staining of HepG2 cells with Brain derived neurotrophic factor antibody (Cat#69354, 1:1,000). Nuclei were stained blue with DAPI; Brain derived neurotrophic factor was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
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