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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | basic transcription factor 3 |
| Gene Symbol: | BTF3 |
| Synonyms: | NACB; BTF3a; BTF3b; BETA-NAC |
| Gene ID: | 689 |
| UniProt ID: | P20290 |
| Clone ID: | 24GB6880 |
| Immunogen: | Recombinant protein of human BTF3 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes the basic transcription factor 3. This protein forms a stable complex with RNA polymerase IIB and is required for transcriptional initiation. Alternative splicing results in multiple transcript variants encoding different isoforms. This gene has multiple pseudogenes.
Images
 | Western blotting analysis using anti-BTF3 antibody (Cat#63681). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-BTF3 antibody (Cat#63681, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-BTF3 antibody (Cat#63681). BTF3 expression in wild-type (WT) and BTF3 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-BTF3 antibody (Cat#63681, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Flow cytometric analysis of BTF3 expression in HepG2 cells using anti-BTF3 antibody (Cat#63681, 1:2,000). Green, isotype control; red, BTF3. |
 | Immunocytochemical staining of HepG2 cells with anti-BTF3 antibody (Cat#63681, 1:1,000). Nuclei were stained blue with DAPI; BTF3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm. |
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