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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | calcium binding protein 39 |
| Gene Symbol: | CAB39 |
| Synonyms: | MO25; CGI-66 |
| Gene ID: | 51719 |
| UniProt ID: | Q9Y376 |
| Clone ID: | 25GB3880 |
| Immunogen: | A synthesized peptide derived from human CAB39/MO25 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
Enables kinase binding activity and protein serine/threonine kinase activator activity. Involved in intracellular signal transduction; peptidyl-serine phosphorylation; and positive regulation of protein phosphorylation. Located in extracellular exosome. Part of cytoplasm and serine/threonine protein kinase complex. Implicated in hepatocellular carcinoma. Biomarker of hepatocellular carcinoma and pancreatic cancer.
Images
 | Western blotting analysis using anti-calcium binding protein 39 antibody (Cat#61819). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-calcium binding protein 39 antibody (Cat#61819, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-calcium binding protein 39 antibody (Cat#61819). Calcium binding protein 39 expression in wild-type (WT) and calcium binding protein 39 (CAB39) knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-calcium binding protein 39 antibody (Cat#61819, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of Calcium binding protein 39 expression in HepG2 cells using anti-Calcium binding protein 39 antibody (Cat#61819, 1:2,000). Green, isotype control; red, Calcium binding protein 39. |
 | Immunocytochemical staining of HepG2 cells with anti-Calcium binding protein 39 antibody (Cat#61819, 1:1,000). Nuclei were stained blue with DAPI; Calcium binding protein 39 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm. |
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