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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | caldesmon 1 |
| Gene Symbol: | CALD1 |
| Synonyms: | CDM; HCAD; LCAD; h-CD; H-CAD; L-CAD; NAG22 |
| Gene ID: | 800 |
| UniProt ID: | Q05682 |
| Clone ID: | 24GB4735 |
| Immunogen: | A synthesized peptide derived from human Caldesmon |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene CALD1 encodes a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and nonmuscle contraction. The conserved domain of this protein possesses the binding activities to Ca(2+)-calmodulin, actin, tropomyosin, myosin, and phospholipids. This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca(2+)-dependent inhibition of smooth muscle contraction. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms.
Images
 | Western blotting analysis using anti-caldesmon 1 antibody (Cat#63256). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-caldesmon 1 antibody (Cat#63256, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-caldesmon 1 antibody (Cat#63256). Caldesmon 1 expression in wild type (WT) and caldesmon 1 (CALD1) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-caldesmon 1 antibody (Cat#63256, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Flow cytometric analysis of caldesmon 1 expression in H9c2 cells using anti-caldesmon 1 antibody (Cat#63256, 1:2,000). Green, isotype control; red, caldesmon 1. |
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