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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | capping actin protein, gelsolin like |
| Gene Symbol: | CAPG |
| Synonyms: | MCP; AFCP; HEL-S-66 |
| Gene ID: | 822 |
| UniProt ID: | P40121 |
| Clone ID: | 25GB3045 |
| Immunogen: | A synthesized peptide derived from human CAPG |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene CAPG encodes a member of the gelsolin/villin family of actin-regulatory proteins. The encoded protein reversibly blocks the barbed ends of F-actin filaments in a Ca2+ and phosphoinositide-regulated manner, but does not sever preformed actin filaments. By capping the barbed ends of actin filaments, the encoded protein contributes to the control of actin-based motility in non-muscle cells. Alternatively spliced transcript variants have been observed for this gene.
Images
 | Western blotting analysis using anti-CAPG antibody (Cat#61619). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CAPG antibody (Cat#61619, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-CAPG antibody (Cat#61619). CAPG expression in wild-type (WT) and CAPG knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-CAPG antibody (Cat#61619, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of CAPG expression in C2C12 cells using anti-CAPG antibody (Cat#61619, 1:2,000). Green, isotype control; red, CAPG. |
 | Immunocytochemical staining of C2C12 cells with anti-CAPG antibody (Cat#61619, 1:1,000). Nuclei were stained blue with DAPI; CAPG was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm. |
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