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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Host Species: | Mouse |
| Isotype: | IgG1 |
| Clonality: | Monoclonal antibody |
| Gene Name: | capping actin protein of muscle Z-line subunit alpha 1 |
| Gene Symbol: | CAPZA1 |
| Synonyms: | CAPZ; CAZ1; CAPPA1 |
| Gene ID: | 829 |
| UniProt ID: | P52907 |
| Clone ID: | 24GB7905 |
| Immunogen: | Recombinant protein of human CAPZA1 |
| Dilution: | WB 1:500-1:2,500; FC 1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
CAPZA1 is a member of the F-actin capping protein alpha subunit family. This gene encodes the alpha subunit of the barbed-end actin binding protein. The protein regulates growth of the actin filament by capping the barbed end of growing actin filaments.
Images
 | Western blotting analysis using anti-CAPZA1 antibody (Cat#63794). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CAPZA1 antibody (Cat#63794, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQTM ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-CAPZA1 antibody (Cat#63794). CAPZA1 expression in wild-type (WT) and CAPZA1 shRNA knockdown (KD) HeLa cells with 40 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-CAPZA1 antibody (Cat#63794, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Flow cytometric analysis of CAPZA1 expression in C2C12 cells using anti-CAPZA1 antibody (Cat#63794, 1:1,000). Green, isotype control; red, CAPZA1. |
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