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| Reactivity: | Human, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | cysteinyl-tRNA synthetase 1 |
| Gene Symbol: | CARS1 |
| Synonyms: | CARS; MDBH; CYSRS; MCDDBH; MGC:11246 |
| Gene ID: | 833 |
| UniProt ID: | P49589 |
| Clone ID: | 25GB2790 |
| Immunogen: | A synthesized peptide derived from human CARS |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene CARS1 encodes a class 1 aminoacyl-tRNA synthetase, cysteinyl-tRNA synthetase. Each of the twenty aminoacyl-tRNA synthetases catalyzes the aminoacylation of a specific tRNA or tRNA isoaccepting family with the cognate amino acid. This gene is one of several located near the imprinted gene domain on chromosome 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian and breast cancers. Alternative splicing of this gene results in multiple transcript variants.
Images
 | Western blotting analysis using anti-cysteinyl-tRNA synthetase 1 antibody (Cat#61550). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-cysteinyl-tRNA synthetase 1 antibody (Cat#61550, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-cysteinyl-tRNA synthetase 1 antibody (Cat#61550). Cysteinyl-tRNA synthetase 1 expression in wild-type (WT) and cysteinyl-tRNA synthetase 1 (CARS1) knockdown (KD) HSHC cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-cysteinyl-tRNA synthetase 1 antibody (Cat#61550, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of Cysteinyl-tRNA synthetase 1 expression in HT-1080 cells using anti-Cysteinyl-tRNA synthetase 1 antibody (Cat#61550, 1:2,000). Green, isotype control; red, Cysteinyl-tRNA synthetase 1. |
 | Immunocytochemical staining of HT-1080 cells with anti-Cysteinyl-tRNA synthetase 1 antibody (Cat#61550, 1:1,000). Nuclei were stained blue with DAPI; Cysteinyl-tRNA synthetase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm. |
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