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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | chromobox 5 |
| Gene Symbol: | CBX5 |
| Synonyms: | HP1; HP1A; HEL25; HP1alpha |
| Gene ID: | 23468 |
| UniProt ID: | P45973 |
| Clone ID: | 25GB2865 |
| Immunogen: | A synthesized peptide derived from human HP1 alpha |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The encoded product is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. The gene has a pseudogene located on chromosome 3. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Images
 | Western blotting analysis using anti-chromobox 5 antibody (Cat#61562). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-chromobox 5 antibody (Cat#61562, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-chromobox 5 antibody (Cat#61562). Chromobox 5 expression in wild-type (WT) and chromobox 5 (CBX5) knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-chromobox 5 antibody (Cat#61562, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of Chromobox 5 expression in HepG2 cells using anti-Chromobox 5 antibody (Cat#61562, 1:2,000). Green, isotype control; red, Chromobox 5. |
 | Immunocytochemical staining of HepG2 cells with anti-Chromobox 5 antibody (Cat#61562, 1:1,000). Nuclei were stained blue with DAPI; Chromobox 5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm. |
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